Composite

Part:BBa_K3450012:Design

Designed by: Priya Sharma Shashaank G.   Group: iGEM20_IISER_Bhopal   (2020-10-23)


Phosphate-Sensitive Kill Switch: Allows E. coli to express E2-IM2 toxin-antitoxin system sensor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 464
    Illegal NheI site found at 487
    Illegal NheI site found at 1825
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 217
    Illegal BglII site found at 1394
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 538
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimized for Escherichia coli


Source

Escherichia coli genome

References